Single cell RNA sequencing revealing gene expression profile of thalamus from mice at the age of 2 months

Single cell RNA transcriptomic expression profile of adult mouse thalamus cells containing astrocytes, oligodendrocytes, mural cells, endothelial cells, etc. The thalamic tissue dissected from two adult wildtype C57BL/6J mice were sequentially digested with papain, protase and dispase in choline chloride solution for preparing single-cell suspensions, and single-cell libraries were generated using 3-labeling kit (V3, 10X Genomics) and sequenced on the Illumina NovaSeq 6000 sequencer.本数据集为包含星形胶质细胞（astrocyte）、少突胶质细胞（oligodendrocyte）、周细胞（mural cell）、内皮细胞（endothelial cell）等细胞类型的成年小鼠丘脑细胞单细胞RNA转录组表达谱。实验取材自2只成年野生型C57BL/6J小鼠的丘脑组织，经解剖分离后在氯化胆碱溶液中依次使用木瓜蛋白酶（papain）、蛋白酶（protease）及分散酶（dispase）酶解制备单细胞悬液；随后采用3'端标记试剂盒（V3，10X Genomics）构建单细胞文库，并于Illumina NovaSeq 6000测序仪上完成测序。