Fig 3 – figure supplement 1: Drop in delta power and time spent awake do not correlate with mEPSC amplitudes or frequency

(A) Schematic showing how drop in delta power is calculated. Delta drop was calculated as percentage drop from sleep epochs that occurred in the window ZT23.5-1.5 to the last hour of sleep right before sacrificing animals. Peak delta power after extended wake at t1 is normalized to the baseline recovery period of t2. (B) mEPSC amplitude after a sleep dense epoch, plotted against normalized drop in delta power during that epoch. Black line = linear fit; R2 value and p value included on plot. Cell averages from different regions are indicated in legend. (C) Same as B) but for mEPSC frequency. (D) Schematic illustrating time spent awake in preceding 4 hours of a wake dense epoch. (E) mEPSC amplitude after a wake dense epoch, plotted against proportion of time spent awake in previous 4 hours. Cell averages from different regions are indicated in legend; light period recordings in green, dark period recordings in Red. (F) Same as (E) but for mEPSC frequency.

(A) 示意图阐明δ功率（delta power）降幅的计算方法：δ功率降幅以授时时间（Zeitgeber Time）23.5-1.5区间内的睡眠时段为基准，计算其相较于动物安乐死直前最后一小时睡眠时段的百分比降幅。延长觉醒后于t1时刻达到的峰值δ功率，需以t2时段的基线恢复期进行归一化处理。(B) 将睡眠密集时段后的微小兴奋性突触后电流（miniature excitatory postsynaptic current, mEPSC）幅值，与该时段内δ功率的归一化降幅进行散点绘图。图中黑色实线为线性拟合线，标注有决定系数R²与p值。图例中标注了不同脑区的细胞平均数据。(C) 与(B)内容一致，但针对微小兴奋性突触后电流频率。(D) 示意图展示觉醒密集时段前4小时内的总觉醒时长。(E) 将觉醒密集时段后的微小兴奋性突触后电流幅值，与该时段前4小时内的觉醒时长占比进行散点绘图。图例中标注了不同脑区的细胞平均数据；光照期记录以绿色标注，黑暗期记录以红色标注。(F) 与(E)内容一致，但针对微小兴奋性突触后电流频率。