Functional characterization of HCA1, HCA2, and HCA3 orthologs.
收藏Figshare2019-05-23 更新2026-04-29 收录
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https://figshare.com/articles/dataset/Functional_characterization_of_HCA_sub_1_sub_HCA_sub_2_sub_and_HCA_sub_3_sub_orthologs_/8175695
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CHO-K1 cells were transfected with receptor constructs and cAMP accumulation was determined. The basal cAMP level of mock-transfected CHO-K1 stimulated with 2 μM forskolin (control) was 41.1 ± 1.9 nmol/well in DMEM and 13.8 ± 1.3 nmol/well in HBSS and set 100%. Analyses of HCA1 were carried out in DMEM. HCA2 and HCA3 were assayed in HBSS. Emax values are shown as % of mock-transfected CHO-K1 stimulated with 2 μM forskolin in absence of agonist. Emax and EC50 values were determined from concentration-response curves using GraphPad Prism. A general explanation of EC50 and Emax values is provided in the results section Functional hominoid HCAR analyses with known endogenous agonists. Data is given as mean ± SEM (basal and Emax) and geometric mean with 95% confidence interval (EC50) of at least three independent experiments each performed in triplicates.
创建时间：
2019-05-23
