RECOMBINANT DNA CONSTRUCTS and METHODS FOR THE MODULATION OF THE EXPRESSION OF A GENE-ALVO

This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA， by interfering with the binding of the small RNA to its target gene. More specifically， this invention discloses recombinant DNA constructs encoding cleavage blockers， 5-modified cleavage blockers， and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes， miRNA decoys， cleavage blockers， 5-modified cleavage blockers， and translational inhibitors， as well as methods for their use， and transgenic eukaryotic cells and organisms containing such constructs. This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA， by interfering with the binding of the small RNA to its target gene. More specifically， this invention discloses recombinant DNA constructs encoding cleavage blockers， 5-modified cleavage blockers， and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes， miRNA decoys， cleavage blockers， 5-modified cleavage blockers， and translational inhibitors， as well as methods for their use， and transgenic eukaryotic cells and organisms containing such constructs.

本发明提供了重组DNA构建体（recombinant DNA constructs）及相关方法，可通过干扰小RNA（small RNA）与其靶基因的结合，实现对受该小RNA调控的靶基因表达的调控。更具体而言，本发明公开了编码切割阻断剂、5'修饰切割阻断剂以及可用于调控靶基因表达的翻译抑制剂的重组DNA构建体，及其使用方法。此外，本发明还公开了可用于设计重组DNA构建体的miRNA（microRNA）靶点，此类构建体包括无miRNA应答的转基因（miRNA-unresponsive transgenes）、miRNA诱饵（miRNA decoys）、切割阻断剂、5'修饰切割阻断剂与翻译抑制剂，同时还公开了它们的使用方法，以及包含此类构建体的转基因真核细胞与生物体。
本发明提供了重组DNA构建体（recombinant DNA constructs）及相关方法，可通过干扰小RNA（small RNA）与其靶基因的结合，实现对受该小RNA调控的靶基因表达的调控。更具体而言，本发明公开了编码切割阻断剂、5'修饰切割阻断剂以及可用于调控靶基因表达的翻译抑制剂的重组DNA构建体，及其使用方法。此外，本发明还公开了可用于设计重组DNA构建体的miRNA（microRNA）靶点，此类构建体包括无miRNA应答的转基因（miRNA-unresponsive transgenes）、miRNA诱饵（miRNA decoys）、切割阻断剂、5'修饰切割阻断剂与翻译抑制剂，同时还公开了它们的使用方法，以及包含此类构建体的转基因真核细胞与生物体。