Methionine metabolism regulates T cell fate by KCa3.1 activity (Cut&Run)

We found that methionine availibility during T cell activation regulates rapid NFAT1 activation, thereby governing T cell fate. Further, acute methionine limitation didn't affect global histone methylation status stating the crucial role of methioinine during early TCR activation. Overall design: Cut&Run analysis of 18 samples. NFAT1 binding sites along with Histone modification status of H3K27me3 and H3K4me3 were analyzed. Two conditions (0.1mM and 0.03mM methionine) were used with 2 replicates/condition were used for NFAT1 analysis and 3 replicates/condition were used for Histone modification analysis.