Assigning function to natural allelic variation via dynamic modeling of gene network induction

The purpose of the experiment is to quantify the dynamic of transcriptional response to galactose exposure by flow cytometric analysis of single yeast cells expressing a GFP reporter system.
Conclusion:
Conclusion are published in the associated research article.
Notes:
We aim i) to describe the dynamical gal response of isogenic strains except for GAL3 (FIG1. Experiments 20152109 and 20151019), ii) to infer GAL3-dependant parameters associated with GAL3BY, GAL3Y12 and GAL3YJM978 alleles (FIG3. Experiments 20160429 and 20160509), iii) to predict the behaviour of GAL3BY, GAL3Y12 and GAL3YJM978 alleles at [gal]=0.2% (FIG3. Experiment 20160826), iv) to infer GAL3-dependant parameters associated with GAL3BY, GAL3DBVPG1788, GAL3NCYC361 and GAL3BY-H352D alleles (FIG4 and FIG6. Experiment 20161202) and v) to infer GAL3-dependant parameters associated with GAL3BY, GAL3YJM321, GAL3YJM428 and GAL3BC187 alleles (FIG5. Experiment 20161130). Experiments 20151019 and 20150921: GY145 is a control strain without GFP transcriptional reporter.