Supplementary file 4, Expression level shown as FPKM (Fragments Per Kilobase of transcript/gene per Million mapped reads)

This study focuses on understanding how plants, specifically Arabidopsis thaliana, respond to two major pollutants, lead (Pb) and vanadium (V) found in oil contaminated land. While the effects of these pollutants on animals are well-studied, their impact on plants is not as clear. To fill this gap, we exposed Arabidopsis seedlings to Pb and V and analyzed the changes in gene expression using advanced sequencing and validated the data using RT PCR technique. The results showed that exposure to Pb and V led to significant changes in gene expression in Arabidopsis seedlings compared to untreated samples. Through Gene Ontology and pathway analysis, we identified several biological processes and pathways associated with the differentially expressed genes. Overall, this investigation sheds light on the molecular mechanisms underlying the toxicity and tolerance of Arabidopsis to Pb and V, providing valuable insights into how plants respond to heavy metal stress.

本研究聚焦于解析植物——尤其是拟南芥（Arabidopsis thaliana）——对石油污染土壤中两种主要污染物铅（Pb）与钒（V）的响应机制。尽管学界对这类污染物在动物体内的影响已有充分研究，但它们对植物的作用效应仍不甚明晰。为填补这一研究空白，本研究将拟南芥幼苗暴露于铅与钒环境中，并借助高通量测序技术分析基因表达变化，同时通过逆转录聚合酶链式反应（RT PCR）对测序数据进行验证。结果显示，与未处理对照组相比，铅与钒暴露会使拟南芥幼苗的基因表达发生显著改变。通过基因本体（Gene Ontology）富集分析与通路富集分析，本研究鉴定出多个与差异表达基因相关的生物学过程及通路。综上，本研究阐明了拟南芥应对铅与钒毒害及耐受的分子机制，为解析植物响应重金属胁迫的机制提供了极具价值的参考视角。