File S1 - Biosynthesis of Taxadiene in Saccharomyces cerevisiae : Selection of Geranylgeranyl Diphosphate Synthase Directed by a Computer-Aided Docking Strategy

Contains the following files. Figure S1, (a) 1H NMR spectra of taxadiene. 400 MHz, CDCl3: 5.3(t, J = 4.8 Hz, 1H), 2.04–1.90 (m, 14H), 1.61 (s, 6H), 1.53 (s, 9H). (b) 1C NMR spectra of taxadiene. 100 MHz, CDCl3: 124.40, 124.19, 123.79, 123.29, 59.40, 39.73, 39.70, 39.57, 26.77, 26.63, 26.33, 25.70, 17.69, 16.29, 16.02. Figure S2, Production of taxadiene by engineered S. cerevisiae. This strain was cultivated in SD medium with 2% glucose for 66 hour. A: GC-MS analysis of n-Hexane extracts. B: Mass spectra of taxadiene. Figure S3, (a) Western blotting of different GGPPS during exponential growth of yeast. (b) Relative expression of different ggpps analyzed by real-time PCR. Figure S4, Cell mass (OD600) and taxadiene production of SyBE_001115 under different airflow rates. Table S1, Primers used in constructing procedure. Table S2, Homology modeling templates information. (DOCX)