Research data supporting 'Genome-wide CRISPR screening identifies new regulators of glycoprotein secretion'

Files S1 - S6 contain underlying data for figures published in the paper. S1: sgRNA counts of unsorted and sorted population from the CRISPR screen detailed in the paper, which aimed to identify genes that regulate glycoprotein secretion. Sheet 1 is a table of sgRNA counts, including the gene name that each sgRNA targets. Sheet 2 includes full descriptions of the populations. S2: MaGECK (version 0.5.7) analysis of sgRNA counts from the CRISPR screen. Both MaGECK-MLE and MaGECK-RRA results are shown. False discovery rates (FDR) from this table were used to create figure 1C-E S3: Raw FCS files from flow cytometry staining of sorted samples, used to create figure 1B. S4: Raw chemiluminescence data, used to create figure 2. S5: Unedited image files used in Figures 3D, 4 and 5. S6: Data output from Cell Profiler, showing total number of cells identified per image and number of cells classified as having fragmented or intact Golgi. E1-E4 is extended data for figures and tables published in the paper. E1: Figure of all genes analysed for having fragmented Golgi; top hits from this talbe are shown in Figure 3C. Percentage of cells with fragmented Golgi for all of the hits screened in the tertiary screen. As in Figure 3C, hits are arranged alphabetically and coloured by cell count, with darker blue spots representing more confluent wells. E2: Sequence of P5 primers used for PCR amplification of sgRNA. E3: Sequence of P7 primers used for PCR amplification of sgRNA. E4: Details of the siGenome pooled siRNA library used in secondary screening.

文件S1至S6为本论文已发表图表的原始支撑数据。
S1：本论文所述CRISPR筛选中未分选与分选细胞群体的sgRNA（单引导RNA，single-guide RNA）计数数据，该筛选旨在鉴定调控糖蛋白分泌的基因。工作表1为sgRNA计数表，包含每条sgRNA靶向的基因名称；工作表2包含各细胞群体的完整说明。
S2：基于本次CRISPR筛选sgRNA计数数据的MaGECK（版本0.5.7）分析结果，包含MaGECK-MLE与MaGECK-RRA两种分析模块的输出，该表格中的错误发现率（FDR，false discovery rate）被用于绘制图1C至1E。
S3：分选样本经流式细胞术染色后的原始FCS（Flow Cytometry Standard）文件，用于绘制图1B。
S4：原始化学发光数据，用于绘制图2。
S5：用于制作图3D、图4与图5的未编辑原始图像文件。
S6：Cell Profiler的输出数据，展示每张图像中识别出的总细胞数，以及被分类为高尔基体（Golgi）碎片化或完整的细胞数量。
E1至E4为本论文已发表图表与表格的补充数据。
E1：所有经高尔基体碎片化分析的基因汇总表格，该表中的核心筛选命中基因已展示于图3C；同时包含三级筛选中所有命中基因对应的高尔基体碎片化细胞百分比。与图3C一致，命中基因按字母顺序排列，并以细胞计数进行着色，深蓝色斑点代表汇合度更高的培养孔。
E2：用于sgRNA聚合酶链式反应（PCR，Polymerase Chain Reaction）扩增的P5引物序列。
E3：用于sgRNA聚合酶链式反应扩增的P7引物序列。
E4：二级筛选中使用的siGenome混合siRNA文库的详细信息。