GNPS - Actinomycetes from Himalaya Hm125, Hm151 and S. coelicolor

Actinomycete strains isolated from the Himalaya mountains were grown for 7 days on minimal medium with 0.5% mannitol and 1% glycerol and extracted in 50 ml ethyl acetate overnight at room temperature. Samples were run on Agilent 1290 UHPLC coupled to a Bruker Daltonics mictoTOF-QII equipped with standard electrospray source. The instrument was fitted with a Kinetex C18 column (50cm x 2.1 mm, 100A). Strain Hm125 was cocultured with Hm151 and Streptomyces coelicolor and results were compared to single cultures.