Additional file 1 of Long-insert sequence capture detects high copy numbers in a defence-related beta-glucosidase gene βglu-1 with large variations in white spruce but not Norway spruce

Additional file 1. Supplementary Table 1. Provenances of the samples analysed in this study for P. abies and P. glauca. Supplementary Table 2. Details of the genomic regions targeted by the sequence capture bait set Picea_hung_p1.0. Supplementary Figure 1. Alignment rates of the probe set Picea_hung_p1.0 on the reference genomes of 5Picea species. Supplementary Table 3. Statistics of local genome assembly, transcriptome assembly, and gene models ofP. abies and P. glauca. Supplementary Figure 2. Syntenic relationship of 7 complete or near-complete Pgβglu-1 gene forms in P. glauca with the reference Pgβglu-1 gene model (KJ780719.1). Supplementary Figure 3. Phylogenetic tree of 139 βglu-1-like gene models in the local assemblies of P. abies (red tips) and P. glauca (blue tips) and their protein multiple sequence alignment. The bold tip labels denote the complete and near-complete gene forms ofβglu-1. Supplementary Table 4. Details of the high-confidence single-copy genes shared by P. abies andP. glauca. Supplementary Table 5. Analysis of variance (ANOVA) table of the total copy number of Paβglu-1 in P. abies. Supplementary Table 6. Analysis of variance (ANOVA) table of the total copy number of Pgβglu-1 in P. glauca. Supplementary Figure 4. (a) Standard curve of Pgβglu-1 synthetic oligomer. (b) Copy number of Pgβglu-1 in P. glauca across their provenances estimated by qPCR. The error bar shows mean± 1 standard error. Supplementary Table 7. TukeyHSD pairwise comparison table of the total copy number of Pgβglu-1 in P. glauca estimated with sequence analysis. Supplementary Table 8. TukeyHSD pairwise comparison table of the total copy number of Pgβglu-1 in P. glauca estimated with qPCR. Supplementary Table 9. Analysis of variance (ANOVA) table of the total copy number of PaUgt5b in P. abies. Supplementary Table 10. Analysis of variance (ANOVA) table of the total copy number of PgUgt5 in P. glauca.

附加文件1。补充表1：本研究中分析的挪威云杉（P. abies）与白云杉（P. glauca）样本的种源信息。
补充表2：序列捕获诱饵套件Picea_hung_p1.0靶向的基因组区域详情。
补充图1：探针套件Picea_hung_p1.0在5个云杉属物种参考基因组上的比对率。
补充表3：挪威云杉与白云杉的本地基因组组装、转录组组装及基因模型统计信息。
补充图2：白云杉中7个完整或近完整的Pgβglu-1基因亚型与参考Pgβglu-1基因模型（KJ780719.1）的共线性关系。
补充图3：挪威云杉（红色分支末端）与白云杉（蓝色分支末端）本地组装组中139个βglu-1样基因模型的系统发育树及其蛋白质多序列比对结果。加粗的分支末端标签代表βglu-1的完整及近完整基因亚型。
补充表4：挪威云杉与白云杉共有的高置信度单拷贝基因详情。
补充表5：挪威云杉中Paβglu-1总拷贝数的方差分析（ANOVA）表。
补充表6：白云杉中Pgβglu-1总拷贝数的方差分析（ANOVA）表。
补充图4：(a) Pgβglu-1合成寡聚核苷酸的标准曲线；(b) 通过实时定量PCR（qPCR）估算的不同种源白云杉中Pgβglu-1的拷贝数。误差棒表示平均值±1倍标准误。
补充表7：通过序列分析估算的白云杉Pgβglu-1总拷贝数的TukeyHSD两两比较表。
补充表8：通过实时定量PCR（qPCR）估算的白云杉Pgβglu-1总拷贝数的TukeyHSD两两比较表。
补充表9：挪威云杉中PaUgt5b总拷贝数的方差分析（ANOVA）表。
补充表10：白云杉中PgUgt5总拷贝数的方差分析（ANOVA）表。