In silico (BLAST) search for primer sequences from F. virguliforme in the IGS sequences of other Fusarium spp. and other fungi expected in fields with corn and soybean production.

aA GenBank number was provided if the search provided a reference.bThe blast search was conducted if a GenBank entry was found. The highest value of the maximum score is given.“(Y)” The 3′ end of the forward and reverse primers at least matches 5 bases of the shown sequences separately, which means the possible success of amplification for the sequence in the database by Fv-IGS primer.“(N)” The 3′ end of the forward and reverse primers does not match at least 5 bases of the shown sequences separately, which means very low possibility of successful amplification for the sequence in the database by Fv-IGS primer.“(NN)” The possible amplification size is too big (>5000 bp).cThe hit sequences were in IGS region.dThe hit sequences were not in IGS region, and they were in other nuclear regions.“N/A” not applicable because no matches were deposited in GenBank.