ShuTu: Rat CA1 pyramidal neuron 63X (Parts 1 - 12)

Here we provide images of a rat CA1 pyramidal neuron (63X) for practicing reconstruction using the ShuTu software platform. The cell was imaged by Ching-Lung Hsu in the Spruston Lab. Due to large sizes of the images files, the data for some neurons are split into multiple parts. After downloading and decompressing, the parts should be combined into one directory.ShuTu (Chinese for "dendrite") is a software platform for semi-automated reconstruction of neuronal morphology. It is designed for neurons stained following patch-clamp recording and biocytin filling/staining. With an additional preprocessing step, it can also handle fluorescence images from confocal stacks.
Even when there is only a single neuron in the imaged volume, automated reconstruction is tricky, because of background. Thus, ShuTu was developed to facilitate a two-step process: automated reconstruction, followed by manual correction. The software uses sophisticated algorithms to perform the automated reconstruction, as well as a convenient user interface to facilitate efficient completion of the reconstruction via manual annotation.

本数据集提供大鼠CA1锥体神经元（63倍放大）的成像图像，用于依托ShuTu（意为“树突”）软件平台开展神经元重建练习。该神经元图像由Spruston实验室的徐青龙（Ching-Lung Hsu）拍摄采集。由于图像文件体积较大，部分神经元的数据集被拆分为多个分卷。用户下载并完成解压后，需将所有分卷合并至同一目录下。
ShuTu（意为“树突”）是一款用于神经元形态半自动化重建的专业软件平台。该软件最初专为经膜片钳记录结合生物胞素填充/染色的神经元样本开发，若增加额外预处理步骤，也可处理共聚焦堆栈获取的荧光图像。
即便成像视野中仅存在单个神经元，受背景噪声干扰，自动化重建仍颇具挑战。为此，ShuTu被设计为支持「自动化重建+人工校正」的两步工作流程：先完成自动化重建，再进行人工修正。该软件搭载精密算法执行自动化重建任务，同时配备便捷的用户界面，可通过人工标注高效完成整个重建流程。