Three-color whole cell LLSM imaging data of ER, H2B, and Lyso over 980 time points during mitosis (Lyso channel)

This dataset includes the three-color whole cell lattice light-sheet microscopy (LLSM) data of ER, H2B, and Lysosomes over 980 time points at 6 sec intervals in a HeLa cell stably expressing calnexin-mEmerald, H2B-mCherry and Lamp1-Halo during mitosis (only Lyso channel here due to the file size restriction, other channels can be found in the same depository with different DOI), which was used to demonstrate SiS-rDL denoising algorithm in our Nature Biotechnology paper (DOI: 10.1038/s41587-022-01471-3). This dataset can be used for non-commercial purposes with proper citations of our NBT paper.