Controlled carbon dioxide enrichment using beer fermentation off-gas results in 89% spinach yield increase

CO2 enrichment is a strategy commonly employed by indoor/vertical farms to improve crop yields. Artificially elevating the CO2 concentration within an enclosed growth space results in improved photosynthetic efficiency, and therefore improved crop growth rates and flavor profiles. Alcohol fermentation provides a steady stream of CO2 that is emitted to the atmosphere as a by-product. To evaluate the feasibility of using fermentation off-gas as a source for indoor carbon dioxide (CO2) enrichment, spinach plants were randomly assigned to 2 growth chambers. The control chamber was maintained under ambient CO2 concentrations, while the treatment chamber was piped onto a flow control system capable of delivering CO2 from a beer fermentation vessel to the growth chamber to maintain CO2 levels at a setpoint of 1200 parts-per-million (ppm). CO2 flow was diverted to the growth chamber when levels were below 1200 ppm, and to an external exhaust when chamber levels were above 1200 ppm, with the flo..., The dataset wass collected by harvesting plants and evaluating them for fresh weight, dry weight, and longest leaf+stem, , # Controlled Carbon Dioxide Enrichment Using Beer Fermentation Off-Gas Results in 89% Spinach Yield Increase

[https://doi.org/10.5061/dryad.15dv41p6f](https://doi.org/10.5061/dryad.15dv41p6f)

## Description of the data and file structure

8 plants were grown in each of 2 chambers (Control, and Treatment). The \"Treatment\" chamber was CO2 enriched using fermentation off-gas. One of the 8 control chamber plants was stunted, and therefore removed from analysis to prevent it from positively biasing the effect of the treatment relative to the control. The removed plant is identified in the data file. Total above ground biomass was harvested and weighed. The longest stem/leaf length was then taken, and finally, plants were dried and weighed for dry weight. 
### Files and variables

#### File: Raw\_Data.csv

**Description:** Raw fresh weight, dry weight, and stem/leaf length data

##### Variables

* Control: 8 plants (7 used for analysis) grown under ambient air conditions
* Treatment: 8 plan...