Pisum sativum cultivar:Lifter and PI240515 Transcriptome or Gene expression

The objective of this project is to utilize recent advances in massively parallel sequencing technology to study the regulation of genes during the S. sclerotiorum and P. sativum host-pathogen interaction. To identify pea resistance genes and Sclerotinia pathogenicity genes we will compare gene expression in parents of a pea recombinant inbred line that is segregating for partial resistance to S. sclerotiorum. The identification of genes involved in partial resistance may lead to the development of markers for marker assisted breeding for resistance to S. sclerotiorum. The expression profiling studies will also provide valuable information on the genetics of the interaction between host and pathogen which may lead to further insights and solutions for developing resistant material not only in pea but in other crops affected by Sclerotinia spp. Additional objectives of this proposal are to provide characterization of genes for improved annotation of the Sclerotinia genome project and gene function discovery for both pea and S. sclerotiorum.

本项目的宗旨在于运用最新的大规模并行测序技术，探讨在菌核病菌（S. sclerotiorum）与黄瓜（P. sativum）的宿主-病原体相互作用过程中基因调控机制。为识别豌豆抗病基因及菌核病菌致病性基因，我们将对比分析具有对菌核病菌部分抗性的豌豆重组自交系的亲本基因表达情况。部分抗性相关基因的鉴定有望推动菌核病菌抗性标记辅助选择育种的发展。此外，表达谱分析还将为揭示宿主与病原体之间遗传学相互作用提供宝贵信息，有助于进一步深入理解和解决不仅针对豌豆，还针对受菌核病菌（Sclerotinia spp.）影响的其它作物抗性材料的研发问题。本提案的附加目标还包括对基因进行表征，以提升菌核病菌基因组项目基因注释的准确性，并发现豌豆及菌核病菌的基因功能。