Sequencing H. hispanica DF60 CRISPR-cas newly acquired spacer sequences and Tm vs TAdm co-culture test genomes

Sequencing analysis of newly acquired spacers detected by PCR after HHPV-2 (MOI~40) virus infestation of DF60, Tm and TAdm mutant strains. Also included are new spacers acquired by TAdm that have not been treated with HHPV2. H. hispanica Tm and TAdm were cultured to log phase and then diluted to the same OD, the two cultures were mixed and inoculated and cultured for 7 days, then transferred to and continued in culture until day 28. Cells were collected from each batch of cultures, centrifuged to extract genomic DNA and analysed by high throughput sequencing.