Behavioural data related to threat-detection strategy in mice

The zipped file <b>a simple threat detection strategy in mice.zip</b> contains seven folders. All the folders contain files in .txt file format.<br>These are: fig1 examples, fig2 speed, fig3 size, fig4 natural stimuli, figS1 defensiveResponses, figS2 trial 1-5, figS3 v014.<br>Folder fig1 examples: contains the .txt files flight S01-20180224-02.txt, freezing S03-20180227-05.txt, and rearing S02-20180601-10.txt.Folder fig2 speed contains 13 sub folders. Folder fig3 size contains 28 sub folders. Folder fig4 natural stimuli contains 8 sub folders. Folder figS1 defensiveResponses contains three sub folders. Folder figS2 trial 1-5 contains 12 .txt filesFolder figS3 v014 contains 10 sub folders.In all the sub folders, the text files are in the following format: S0X-20XXXXXX-XX.txt, where X is a number.<br>Raw behavioural data collected with the infrared touchscreen frame in each figure, have been contained in the particular folder. Each txt file contains the behavioural data of one mouse.The folder “fig1 examples” relates to figure 1, and shows the three remarkable behaviors in response to looming stimuli.The folder “fig2 speed” relates to figure 2, and shows the flight, freezing and rearing responses induced by an expanding shadow with constant speed.The folder “fig3 size” relates to figure 3, and shows the responses induced by expanding shadows with different changes in expanding size.The folder “fig4 natural stimuli” relates to figure 4, and shows the responses induced by simulative natural visual stimuli.The folder “figS1 defensiveResponses” relates to additional file 1, figure S1, and shows the properties of flight, freezing and rearing responses in mice.The folder “figS2 trial 1-5” relates to additional file 2, figure S2, and shows the behavioural tendencies from the first to fifth looming stimulus.The folder “figS3 v014” relates to the additional file 3, figure S3, and shows the responses induced by the 14°/s expanding shadows with different changes in expanding size. <br>The 1st line in each of the text files, represents the beginning time of the recording.The 2nd and 3rd lines are reserved except the last string in the 3rd line, which is a time tag.The 4th to the last lines are touch-input data. Data from left to right are x, y, dwID, dwFlags, dwTime, cxContact, cyContact, respectively.For the definition of the touch-input data, please see:https://docs.microsoft.com/en-us/windows/win32/api/winuser/ns-winuser-touchinput<br><b>Study aims and methodology:</b>Avoiding danger and accessing environmental resources are two fundamental, yet conflicting, survival instincts across species. To maintain a balance between these instincts, animals must efficiently distinguish approaching threats from low-threat cues. However, little is known about the key visual features that animals use to promptly detect such imminent danger and thus facilitate an immediate and appropriate behavioural response.In this study, using a series of looming stimuli with different sizes and expanding speeds, the authors systematically analysed flight, freezing, and rearing behaviour in mice that were induced by these approaching visual cues.Male C57BL6/J mice (Beijing Weitonglihua Corp.) aged 8–10 weeks, were used for all the experiments. The looming test, behavioural analysis and statistical analysis are described in more detail in the related article.<br>

压缩文件<b>小鼠简单威胁检测策略.zip</b>内含7个文件夹。所有文件夹均存储TXT格式文件，分别为：fig1 examples、fig2 speed、fig3 size、fig4 natural stimuli、figS1 defensiveResponses、figS2 trial 1-5及figS3 v014。

fig1 examples文件夹：内含flight S01-20180224-02.txt、freezing S03-20180227-05.txt及rearing S02-20180601-10.txt三个TXT文件。fig2 speed文件夹包含13个子文件夹；fig3 size文件夹包含28个子文件夹；fig4 natural stimuli文件夹包含8个子文件夹；figS1 defensiveResponses文件夹包含3个子文件夹；figS2 trial 1-5文件夹包含12个TXT文件；figS3 v014文件夹包含10个子文件夹。

所有子文件夹内的文本文件均遵循如下命名格式：S0X-20XXXXXX-XX.txt，其中X为数字。

各对应文件夹存储了通过红外触摸屏框架采集的、与对应图表相关的原始行为学数据。每个TXT文件存储单只小鼠的行为学数据。

"fig1 examples"文件夹对应图1，展示了小鼠面对迫近视觉刺激时的三种典型行为。"fig2 speed"文件夹对应图2，展示了恒定速度扩张的阴影诱导产生的逃跑、僵直及直立探究行为。"fig3 size"文件夹对应图3，展示了不同扩张尺寸变化的扩张阴影所诱导的行为反应。"fig4 natural stimuli"文件夹对应图4，展示了模拟自然视觉刺激所诱导的行为反应。"figS1 defensiveResponses"文件夹对应补充材料1（图S1），展示了小鼠逃跑、僵直及直立探究行为的特性。"figS2 trial 1-5"文件夹对应补充材料2（图S2），展示了小鼠在第1至第5次迫近视觉刺激下的行为倾向。"figS3 v014"文件夹对应补充材料3（图S3），展示了以14°/秒速度扩张、且扩张尺寸存在不同变化的阴影所诱导的行为反应。

每个TXT文件的第1行代表记录的起始时间。第2行与第3行预留作他用，但第3行的最后一个字符串为时间标签。第4行至末尾行均为触摸屏输入数据，各列数据从左到右依次为：x坐标、y坐标、dwID、dwFlags、dwTime、cxContact、cyContact。

触摸屏输入数据的具体定义可参考：https://docs.microsoft.com/en-us/windows/win32/api/winuser/ns-winuser-touchinput

<b>研究目的与方法：</b>
规避危险与获取环境资源是所有物种共有的两项基本却相互冲突的生存本能。为在这两种本能间维持平衡，动物必须高效区分迫近的威胁与低威胁线索。然而，目前学界对动物用于快速识别此类迫近危险、从而触发即时恰当行为反应的关键视觉特征仍知之甚少。

本研究通过一系列具备不同尺寸与扩张速度的迫近视觉刺激，系统分析了此类迫近视觉线索诱导小鼠产生的逃跑、僵直及直立探究行为。本研究所有实验均使用8~10周龄的雄性C57BL6/J小鼠（北京维通利华实验动物技术有限公司）。迫近刺激测试、行为学分析及统计学分析的详细方法已在相关论文中予以说明。