MOESM9 of Novel DNA methylation biomarkers show high sensitivity and specificity for blood-based detection of colorectal cancer—a clinical biomarker discovery and validation study

Additional file 9: Table S1. DNA methylation level of C9orf50, KCNQ5 and CLIP4 obtained from Infinium. HumanMethylation450K BeadChip® array data. Table S2. Primer sequences used for bisulfite sequencing of candidate biomarker regions. Table S3. Overview and primer and probe sequences for methylation-specific ddPCRT of the 29 selected biomarker candidates. Table S4. Sensitivities and specificities in plasma from CRC patients and healthy controls (test cohort) for individual markers and the TriMeth test. Table S5. Primer and probe sequences for ddPCR control assays. Table S6. Digital MIQE checklist for authors, reviewers and editors.

附加文件9：表S1。源自Infinium® HumanMethylation450K 微珠芯片（Infinium® HumanMethylation450K BeadChip®）阵列数据的C9orf50、KCNQ5及CLIP4的DNA甲基化水平。
表S2。候选生物标志物区域亚硫酸氢盐测序所用引物序列。
表S3。针对29个筛选出的候选生物标志物的甲基化特异性ddPCRT的概况、引物及探针序列。
表S4。结直肠癌（Colorectal Cancer, CRC）患者与健康对照（测试队列）的血浆样本中，单个标志物及TriMeth检测的灵敏度与特异度。
表S5。液滴数字PCR（ddPCR）对照实验的引物与探针序列。
表S6。供作者、审稿人及编辑使用的数字聚合酶链式反应MIQE核查清单。