Genome-wide RNA sequencing analysis in Il10-/- bone marrow-derived macrophages stimulated by lipopolysaccharide

We report the genome-wide RNA sequencing analysis in Il10-/- bone marrow-derived macrophages (BMDMs) stimulated by lipopolysaccharide (LPS) where IL-10 effect in macrophage inflammatory response was examined in IL-10-deficient BMDMs upon LPS stimulation with addition of exogenous IL-10. Examination of IL-10 effect in bone marrow-derived macrophages (BMDMs) in response to LPS stimulation. To grow bone-marrow derived macrophages (BMDMs), bone marrow cells were flushed from femur and tibia bones of IL-10-deficient mice strain in C57BL/6 background between 6 and 12 weeks of age, and were grown at 37°C in a humidified incubator in RPMI-1640 medium containing L-glutamine, 10% FCS and 30% L929 supernatant (i.e. BMDM growth media) for 7-8 days. The RNA sequencing analysis was performed separately in two groups of samples. Group 1: Il10-/- BMDMs were stimulated for 3 h without (control samples C1 and C2) or with 100 ng/ml of lipopolysaccharide (LPS)(samples C3 and C4), 100 ng/ml of IL-10 (samples D1 and D2) or both (samples D3 and D4). Group 2: Il10-/- BMDMs were stimulated without (control samples K1 and K2) or with 100 ng/ml of LPS (for 6 h: samples K3 and K4; for 12 h: samples K9 and K10), 100 ng/ml of IL-10 (for 6 h: samples K7 and K8; for 12 h: samples K13 and K14) or both (for 6 h: samples K5 and K6; for 12 h: samples K11 and K12).