*The mutant efficiency is calculated by the mutant TA-cloning of PCR products of mixed cells/total sequencing number. The mismatch affected the ZFNs cut-efficiency dramatically. Lowercase letters were
The CRISPR/Cas9 is a new system that can introduce site-specific double strand breaks. In this system, sequence specificity is achieved by 20-nucleotide RNA molecules that guild endonuclease to the s
‘I-SceI+/total’ indicates the fraction of Ade+ survivors that were capable of being re-cleaved by I-SceI, demonstrating that they had used precise NHEJ. ‘Colony Survival’ is the fraction of Ade+ colon