The GPR43-mediated function of eosinophils in asthma

GPR43 is a short-chain fatty acid receptor exerting anti-inflammatory functions in various inflammatory diseases. Even though eosinophils are one of the cell types highly expressing GPR43, the role of GPR43 in regulating eosinophils has not been studied. In this experiment, we induced asthma in control (Epx-Cre/+) mice and eosinophil-specific GPR43-deficient (Epx-Cre/+GPR43fl/fl) mice using house dust mite extracts and compared the immune cell landscape in the asthmatic lungs of each group using single-cell RNA sequencing. Cells were extracted from asthmatic lungs of control and eosinophil-specific GPR43-deficient mice (3 mice for each group) and pooled. Dead cells and FSC(hi)SSC(hi) cells were excluded by fluorescence-activated cell sorting (FACS).