Neuronal development in NEIL3-deficient mice (hippocampus)

brain tissue from all subregions (CA1, CA3, dentate gyrus) of the hippocampal formation was isolated at postnatal day 8 and at an adult age (3-6months old animals) in wildtype and NEIL3-deficient mice (n=4 at p8 and n=3 at adult age). NEILs are DNA glycosylases potentially involved in transcription regulation and hippocampal neuronal maturation. RNA was extracted and sequenced. Wildtype (C57Bl6) and mice deficient in NEIL3 (C57Bl6-background) were used. Tissue was isolated exclusively at baseline, i.e. the animal was taken directly from its homecage without any behavioral intervention本研究从野生型（wildtype）与NEIL3缺陷型（NEIL3-deficient）小鼠的海马结构全部亚区（CA1、CA3、齿状回（dentate gyrus））分离脑组织，取材时间点分别为出生后第8天（postnatal day 8，p8）与成年阶段（3~6月龄），其中p8阶段样本量为n=4，成年阶段样本量为n=3。 NEIL家族蛋白属于DNA糖苷酶（DNA glycosylases），可能参与转录调控与海马神经元成熟过程。 随后提取RNA并进行测序。 本研究使用的小鼠均为C57Bl6遗传背景，包括野生型C57Bl6小鼠以及该背景下的NEIL3缺陷型小鼠。 所有脑组织仅在基线状态下取材，即实验动物直接从饲养笼中取出，未施加任何行为学干预。