Supplementary dataset for Zhu et al. 2024: Tracing the evolutionary history of the temperature-sensing prion-like domain in EARLY FLOWERING 3 shows uniqueness of AtELF3

This record contains a supplementary dataset of sequence alignment files and a GWAS p-value table that support the publication:

Zhu Z, Trenner J, Delker C, Quint M. 2024. Tracing the evolutionary history of the temperature-sensing prion-like domain in EARLY FLOWERING 3 shows uniqueness of AtELF3.

(submitted to Molecular Biology and Evolution)

Included files:

1. datasetFig1_ELF3EEC_tree_20240418_434seqs.fasta

Multiple protein sequences alignment by MUSCLE (Edgar, 2004) of 434 ELF3/EEC protein sequences from 274 plant genomes, supporting the phylogenetic tree in Figure 1.

2. datasetFig2_ELF3EEC_alignment.fasta

Multiple protein sequences alignment by MUSCLE (Edgar, 2004) of 69 ELF3/EEC protein sequences from selected species, supporting Figure 2.

3. datasetFig3_BrassicalesELF3_tree.fasta

Multiple protein sequences alignment by MUSCLE (Edgar, 2004) of 52 ELF3 protein sequences from 44 Brassicales and 8 Poales species, supporting the phylogenetic tree in Figure 3.

4. datasetFig4_At319ELF3_CAAremoved_tree.fasta

Multiple DNA sequences alignment by MUSCLE (Edgar, 2004) of 319 AtELF3 coding sequences (CAA repeats removed) supporting the phylogenetic tree in Figure 3.

5. pvalues_GWA_Portal_polyQ_length_vs_1001genomes_dataset_AMM.csv

For a genome-wide association study (GWAS) the polyQ length of 319 Arabidopsis thaliana accessions was submitted as phenotype to the interactive GWA-Portal (Seren 2018, https://gwas.gmi.oeaw.ac.at). This P-value output file supports Supplemental Figure S10.

Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic acids research 32: 1792-1797

Seren Ü. (2018). GWA-Portal: Genome-Wide Association Studies Made Easy. In: Ristova, D., Barbez, E. (eds) Root Development. Methods in Molecular Biology, Vol 1761. Humana Press, New York, NY.