Platypus PacBio assembly

Files containing PacBio reads selected by BLAST to generate assembly of a complete rDNA (Platypus_rDNA_assembly_reads.fasta.gz), annotated assembly contig (Platypus_sequence_selection_assembly_annotated_contig_3.geneious), annotated complete assembly (Platypus_complete_rDNA_Sequence_Selection_Assembly.geneious) and partial units (Platypus_Sequence_assembly_partial_sequences.geneious).Alignment of the complete contig rDNA unit to a reference gene (GL000220; see Agrawal & Ganley, 2018): (align_platypus_complete_rDNA_unit_seqsel_assembly_with_ref.geneious)Alignment of the complete rDNA unit to a reference gene (Floutsokou et al 2013): (align_platypus_complete_rDNA_unit_seqsel_assembly_with_ref.geneious)Alignment of the partial (28S) rDNA unit to complete rDNA unit from the assembly:(Platypus_partial_rDNA_unit_aligned_with_full_rDNA_unit. genious)Alignments of probable rRNA genes to assembled rRNA genes (alignments_of_probable_platrDNA_with_plat_seqsel_assembly_genes.geneious).Probable 18S rRNA: GenBank accession AJ311679.1Probable 5.8S rRNA: GenBank accession XR_003764684

该数据集包含经 BLAST 筛选的 PacBio 测序读段，用于构建完整的核糖体 DNA (rDNA) 组装 (Platypus_rDNA_assembly_reads.fasta.gz)，以及注释过的组装序列片段 (Platypus_sequence_selection_assembly_annotated_contig_3.geneious)、完整的注释组装 (Platypus_complete_rDNA_Sequence_Selection_Assembly.geneious) 和部分单元 (Platypus_Sequence_assembly_partial_sequences.geneious)。此外，还包括完整 rDNA 片段与参考基因的比对 (GL000220；参见 Agrawal & Ganley, 2018) 的比对结果：(align_platypus_complete_rDNA_unit_seqsel_assembly_with_ref.geneious)；完整 rDNA 片段与参考基因 (Floutsokou et al 2013) 的比对结果：(align_platypus_complete_rDNA_unit_seqsel_assembly_with_ref.geneious)；部分 (28S) rDNA 片段与组装完整 rDNA 片段的比对：(Platypus_partial_rDNA_unit_aligned_with_full_rDNA_unit. genious)；以及推测的核糖体 RNA 基因与组装的核糖体 RNA 基因的比对 (alignments_of_probable_platrDNA_with_plat_seqsel_assembly_genes.geneious)。推测的 18S rRNA：GenBank 访问号 AJ311679.1；推测的 5.8S rRNA：GenBank 访问号 XR_003764684。