The identification of differentially expressed genes between animal and vegetal blastomeres in Xenopus laevis. Xenopus laevis

To identify asymmetrically localized maternal mRNAs along the animal-vegetal axis in cleavage Xenopus embryos, we isolated animal and vegetal blastomeres at 8-cell stage, extracted the maternal mRNA respectively and analyzed them by RNA-seq technology. We identified 43 maternal transcripts significantly enriched in the vegetal region (FDR<0.05) by R/Bioconductor package DESeq Overall design: RNAseq of animal and vegetal blastomeres with 2 biological replicates