Sugar dataset final.xlsx

Single source white clover (Fabaceae: Trifolium repens) pollen pellets collected by Apis mellifera purchased from Ames Farm (Watertown, Minnesota, USA; www.amesfarm.com) and single source rose (Rosaceae: Rosa spp.) pollen collected by Apis mellifera purchased from the China Health Club (Chongqing, China; www.teaherbalshop.com) were used to create artificial diets to raise C. calcarata eggs. Pollen identity and purity were based on vendor claims. Sugar samples were thawed in 50°C water bath for 3 min and then centrifuged at >2,500 rpms for 5 min. Sugar content was measured in degrees Brix by placing 20 mL of the supernatant on a handheld refractometer. Based on the weight of the original pollen ball, we determined the milligrams of sugar in each sample.本研究采用购自美国明尼苏达州沃特敦市Ames农场（www.amesfarm.com）的西方蜜蜂（Apis mellifera）采集的单一来源白车轴草（Fabaceae: Trifolium repens）花粉团，以及购自中国重庆中国健康俱乐部（www.teaherbalshop.com）的西方蜜蜂采集的单一来源玫瑰（Rosaceae: Rosa spp.）花粉，配制人工饲料以饲养C. calcarata卵。花粉的种类与纯度以供应商声明为准。糖样本经50℃水浴解冻3分钟后，以不低于2500转/分钟的转速离心5分钟。取20mL上清液置于手持折光仪中，以白利糖度（degrees Brix）值测定糖含量。根据原始花粉团的重量，推算出每份样本中的糖毫克数。