Proteomic analysis of differentially expressed proteins in chloromethane- and methanol-grown cultures of M. extorquens CM4.

aMaGe database (http://www.genoscope.cns.fr/agc/mage).bProbability-based mowse score calculated using MASCOT software (Matrix Science, London, UK); error refers to mass accuracy; coverage refers to the percentage of the protein sequence covered by the matched peptides.cSpots indicated as “CH3Cl” were only detected in the proteome of M. extorquens CM4 grown with chloromethane. Spots indicated as “+” were more abundant in chloromethane-grown cultures (or less abundant in methanol-grown cultures). Spots indicated as “−” were more abundant in methanol-grown cultures (i.e. less abundant in chloromethane-grown cultures). Factors of differential abundance were defined as follows:++(−−) 2- to 5-fold;+++(−−−) more than 5-fold.dNL, non linear pI range used in 2D-DIGE experiments.eOnly found in strain CM4 (among the 8 Methylobacterium strains for which the complete genome sequence is known; [5], [11]) and localized on plasmid pCMU01.fMultiple spots detected.gMass spectrometry used to discriminate from Mchl_1712 displaying 86% sequence identity at the protein level.hn.d., not detected.iMass spectrometry used to discriminate from Mchl_2317 displaying 96% sequence identity at the protein level.jNo assigned gene name.kMass spectrometry data did not allow us to discriminate between two homologs with 99% sequence identity (Mchl_2669/Mchl_4004).lTandem mass spectrometry identification.