The Effect of Betel Habits on Blood Glucose Levels in Karo ethnic community in Karo District

Burns are damage to the integrity of the normal skin caused by contact with a heat source and are destructive.The body's efforts to carry out the healing process have 3 phases,namely: inflammation,proliferation and maturation.Tissue damage from burns causes inflammation that causes pain.It requires a therapy that can be used in the process of epidermal re-epithelialization and reduce pain due to burns. In previous studies, it has been proven that the extract of the Bangun-bangun leaves (Coleus amboinicus) has effective as a reliever of pain and inflammation after acetic acid induction. In the Karo tribe, it is known as Daun Bangun-bangun (DBB). As a traditional medicine, it is often used directly on the injured skin. Aims: To analyze the effects of DBB extracts in topical gel to reduce pain and accelerate post-burn reepithelialization in rats.Methods: This is an experimental study design with a posttest-only control group design. The sampling method was randomized consisted of 54 rats divided into 9 groups, i.e:I.negative control;II.positive control (Bioplacenton®) and III.Treatment (36% DBB extracts gel). All rats were given second-degree burns except the standard group. The animal was treated 2x in daily. Each group was subdivided into three smaller groups (n = 6) according to the time the lesions were evaluated. Skin tissue was taken on days 3, 14, 21, and data were analyzed to compare the decrease in pain threshold between groups and to calculate the number of leukocyte migration in the healing process. If the data is normally distributed then continued with ANOVA and post hoc Bonferroni tests but, if the data is abnormally distributed, it continued with Kruskal Wallis test. Results: There were decreased of pain in EDBB and positive control (bioplacenton) groups, but in statistically no significantly different in reduced pain (p=0.139). The increasing of number of leucocyte migration compared of EDBB to other groups depend on the day of assessed, but in macroscopis we can see the best healing process in all groups is EDBB group although two other groups almost equally. Conclusion: Bangun-bangun leaves (Coleus amboinicus) extracts gel could reduce pain and accelerate post-burn reepithelialization in rats.

烧伤是指接触热源后造成的正常皮肤完整性破坏，属于损伤性病变。机体启动的愈合过程可分为三个阶段，即炎症期、增殖期与成熟期。烧伤引发的组织损伤会触发炎症反应并伴随疼痛，因此需要一种可用于表皮再上皮化过程、并能缓解烧伤疼痛的治疗手段。既往研究证实，邦邦叶（Bangun-bangun leaves，学名Coleus amboinicus）提取物在乙酸诱导模型中可有效缓解疼痛与炎症。在卡罗族传统中，该植物被称为邦邦叶（Daun Bangun-bangun，简称DBB），作为传统药物常直接敷于受损皮肤。研究目的：分析外用邦邦叶提取物凝胶对大鼠烧伤后疼痛缓解与再上皮化加速的效果。研究方法：本研究采用仅设后测的对照实验设计。通过随机抽样纳入54只大鼠，先分为3个大组，再根据皮损评估时间点将每个大组细分为3个亚组，共9组：Ⅰ. 阴性对照组；Ⅱ. 阳性对照组（Bioplacenton®）；Ⅲ. 治疗组（36%邦邦叶提取物凝胶）。除标准组外，所有大鼠均构建二度烧伤模型。每日对大鼠进行2次给药处理，分别于第3、14、21天获取皮肤组织样本。数据分析环节，若数据符合正态分布，则采用单因素方差分析（ANOVA）结合邦费罗尼（Bonferroni）事后检验；若数据不符合正态分布，则采用克鲁斯卡尔-沃利斯（Kruskal-Wallis）检验。研究结果：邦邦叶提取物凝胶组与阳性对照组（Bioplacenton®）均能缓解大鼠疼痛，但两组的疼痛缓解程度无统计学差异（p=0.139）。邦邦叶提取物组的白细胞迁移数量变化与其他组的对比结果随评估时间点有所不同，但宏观观察可见，尽管其余两组愈合效果相近，邦邦叶提取物组的愈合进程在所有组别中表现最优。研究结论：邦邦叶（Coleus amboinicus）提取物凝胶可有效缓解大鼠烧伤后疼痛，并加速烧伤后再上皮化进程。