RNA-seq analysis of neonatal mouse cochlear hair cells

This study examined transcripts that are enriched in neonatal mouse cochlear hair cells. Hair cells were purified by FACS sorting for GFP fluorescence from the cochleas of transgenic mice in which the endogenous Atoh1 gene was fused with GFP Two replicates of GFP+ hair cells were compared with all other cochlear cell types that were GFP-本研究针对新生小鼠耳蜗毛细胞中富集的转录本展开分析。研究人员从内源Atoh1基因（Atoh1）与绿色荧光蛋白（Green Fluorescent Protein, GFP）融合的转基因小鼠耳蜗中，通过荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）基于GFP荧光信号纯化得到毛细胞。将两份GFP阳性（GFP+）毛细胞的重复样本，与所有其余GFP阴性（GFP-）的耳蜗细胞类型进行对照比较。