Cohesion is established during DNA replication utilising chromosome associated cohesin rings as well as those loaded de novo onto nascent DNAs

Cohesin is established by two parallel pathways during DNA replication. Chromosome associated cohesin is converted to cohesive structures and this depends on four protein associated with the replisome; Tof1/Csm3, Ctf4 and Chl1 (TCCC). In TCCC mutants, chromosome associated cohesin is evicted from the DNA during S phase.黏连蛋白（Cohesin）在DNA复制过程中通过两条平行通路完成建立。结合于染色体的黏连蛋白会转化为黏连结构，这一过程依赖于四种与复制体（replisome）相关的蛋白质：Tof1/Csm3、Ctf4与Chl1（合称TCCC）。在TCCC突变体中，与染色体结合的黏连蛋白会在S期从DNA上被移除。