Eric A. Vitriol, Andrea C. Uetrecht, Feimo Shen, Ken Jacobson, James E. Bear (2011) CIL:25822, Rattus norvegicus, fibroblast. CIL. Dataset

Chromophore-assisted laser inactivation (CALI) inactivates target proteins by generating reactive photoproducts such as reactive oxygen species (ROS) by intense irradiation of photosensitizer chromophores, such as EGFP. Capping protein (CapZ, abbreviated CP throughout) is an obligate heterodimer of alpha and beta subunits and is the predominant actin filament capping protein in most cells. Rat2 cells deficient in endogenous CP, but rescued with EGFP-CP (KDR cells), were subjected to CALI. After administration of a 100-ms dose of 1.5 mW/[μm]2 laser light, KDR cells exhibited dynamic changes on the dorsal surface of the cell, forming numerous protrusions as seen in the DIC time-lapse movie (CIL# 25821). This is a movie of a zoomed area of the irradiated region from CIL# 25821 and control movies are CIL#s 25823, 25824. This movie is Movie2 in Supporting Information and still images are in Fig 2B, top panels, in Proc Natl Acad Sci (2007) 104: 6702-6707.

色团辅助激光失活技术（Chromophore-assisted laser inactivation, 简称CALI）通过强光照射光敏色素色团，如EGFP，产生如活性氧（Reactive oxygen species, 简称ROS）等反应性光产物，从而实现对目标蛋白的失活。帽状蛋白（Capping protein，简称CapZ，全称Capping protein，以下简称为CP）是一种由α和β亚基组成的必需性异源二聚体，是大多数细胞中主要的肌动蛋白丝帽状蛋白。对内源性CP缺乏但经EGFP-CP（KDR细胞）挽救的Rat2细胞进行了CALI处理。在给予100毫秒、1.5毫瓦每平方微米的激光光剂量后，KDR细胞在细胞背表面表现出动态变化，形成如DIC时间推移电影（CIL# 25821）中所示的大量突起。此电影为CIL# 25821中照射区域放大区域的影像，对照电影分别为CIL#s 25823和25824。此电影为辅助信息中的Movie2，而静态图像则位于《美国国家科学院院刊》（Proc Natl Acad Sci，2007年第104卷，第6702-6707页）的图2B顶部面板中。