Live widefield JF635-HaloTag-NLS stage 5 embryo

Wide field imaging in the anterior region of a live stage 5 Drosophila melanogaster embryo expressing HaloTag-NLS. The HaloTag-NLS protein is unstable and degraded after translation. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 50 ms. Video is played at 10 frames per second.

对表达HaloTag-NLS的活体5期黑腹果蝇（Drosophila melanogaster）胚胎的前端区域进行宽场成像。该HaloTag-NLS蛋白性质不稳定，翻译完成后即发生降解。于产卵后2小时，向该胚胎注射与HaloTag配体连接的JF635。使用633 nm激光对该胚胎实施落射照明，并以50 ms的曝光时长采集视频。视频以每秒10帧的速率播放。