C-section delivery related high sensitivity to inflammation in mice is unchained by gut barrier-microbiota imbalances in the early life

The main goal of this study is to decipher the role of CSD in the early-life gut microbiota-barrier alterations and its links with later-life risks of intestinal inflammation Total RNA was isolated from the colon samples using RNeasy Mini Kit, according to manufacturer's instructions. The RNA integrity was verified in Bioanalyzer 2100 with RNA 6000 Nano chips (Agilent Technologies). Only samples that have RNA. To identify the differential expressed genes between CSD and VD samples, we fit a linear model for each gene using the eBayes function from the limma R package, setting the sex and the delivery mode as fixed effects integrity >9 were used for the study.