Maximum likelihood analysis

Maximum likelihood analysis was undertaken using RAxML on the BlackBox server for the combined six gene, 4118 character, 36 taxa dataset. COI, H3 and XPNPEP3 partitioned by codons and ITS1, 5.8S, ITS2 and 28S partitioned individually, with the GTR + G model used for all genes. This analysis resolved the genera Moggridgea and Bertmainius as reciprocally monophyletic, with M. rainbowi from KI clearly embedded within the African Moggridgea lineage and sister to M. intermedia (bootstrap value 100) (Fig 1). Furthermore, M. rainbowi formed a monophyletic group, but showed phylogeographic structure, with haplotypes reflecting the two geographic locations (Western River and American River). The MrBayes analysis of the same dataset produced a completely concordant tree.

本研究针对由6个基因、4118个性状位点、36个分类单元组成的联合数据集，采用RAxML软件在BlackBox服务器上开展最大似然分析：其中COI、H3与XPNPEP3按密码子分区，ITS1、5.8S、ITS2及28S单独分区，所有基因均采用GTR + G模型。此次分析结果显示，莫格里奇蛛属（Moggridgea）与伯特迈厄斯蛛属（Bertmainius）互为单系群，采自KI的彩虹莫格里奇蛛（M. rainbowi）明确嵌入非洲莫格里奇蛛支系之中，且与中间莫格里奇蛛（M. intermedia）构成姊妹群，自举支持值为100（图1）。此外，彩虹莫格里奇蛛（M. rainbowi）自身构成单系群，但呈现出系统地理学分化结构，其单倍型与Western River和American River两个采样地理位点相对应。针对同一数据集的MrBayes分析得到了完全一致的系统发育树。