Genome-wide maps of deaminated CPDs (dCPDs) in yeast genomic DNA in vitro

Here, we describe a genome-wide map of uracil lesions arising form deaminated UV-induced cyclobutane pyrimidine dimers (CPDs) in yeast (Saccharomyces cerevisiae) genomic DNA that were UV-irradiated and deaminated as naked DNA in vitro. Deaminated CPDs (dCPDs) were mapped at single nucleotide resolution across the yeast genome using the new dCPD-seq method. We used these data to analyze CPD deamination in different trinucleotide sequence contexts. We used the new dCPD-seq method to map cytosine-containing UV-induced cyclobutane pyrimidine dimers (CPDs) that had undergone deamination to uracil. Deaminated CPDs were mapped at single nucleotide resolution across the yeast genome in UV-irradiated yeast genomic DNA that was deaminated for 0, 6, 24, or 48 hours (hr) in solution in vitro.