Information S1 - Protein Variants Form a System of Networks: Microdiversity of IMP Metallo-Beta-Lactamases

Table S1. Protein names, gene identifers and mutation profiles of all IMP sequence used in the construction of phylogenetic trees and protein networks. Gene identifiers were taken from (http://www.lahey.org) where available. Table S2. Mutations shared by all variants of the respective network in comparison to the reference sequence IMP-1. Table S3. Triplets of IMP variants with predicted missing variant to complement the triplet to a quartet. Table S4. Pairs of IMP variants which differ by 2 mutations with no know intermediary variants. Intermediary variants for possible pathways were predicted. Table S5. Predicted S262G and V67F variants. Figure S1. The distance matrix and a network representation of a triplet of sequences consisting of a wild type and two single mutants thereof (mutant 1 and mutant 2). Figure S2. The distance matrix of a quartet of sequences consisting of a wild type, two single mutants thereof (mutants1 and 2), and the respective double mutant. Figure S3. Left: Network representation of a quartet of sequences consisting of a wild type, two single mutants thereof (mutants1 and 2), and the respective double mutant (derived from the distance matrix in Figure S2). Right: the four sequences cannot be represented by a binary tree assuming additivity of distances. Figure S4. From a network of four variants of known sequence (black), three variants (red) can be predicted that are expected to be functional and to exist in the biosphere. Figure S5. From the network constructed in Figure S4, four further mutants (green) can be predicted that are expected to be functional and to exist in the biosphere. (PDF)