Effects of fenretinide on proliferation and apoptosis levels of ESFT cells in normoxia and hypoxia over 48 hours.

aThis is the slope of the graph of log CVC fluorescence vs. time. A full description of the analyses is given in the text. bApoptosis was assessed by Annexin-V/PI staining, acquired by FACS and analysed using CellquestPro™ software. The percentage value shown is the sum total of cells that stained positive for Annexin-V alone, Annexin-V+PI together, and PI alone. The data were compared statistically using the non-parametric Mann-Whitney-Wilcoxon rank sum test. Values shown are mean ± SEM of n = 6 determinations. *Value in hypoxia is significantly different from normoxia at p value indicated.