10,000 unique barcodes of length 18 were generated with padding option set to 1.

Then 200,000 barcoded reads of length 18 are generated and errors are introduced with 2% probability, substitutions, insertions and deletions are allowed. With k-mer length of 6, all reads can be demultiplexed correctly in less than half a minute, if the user uses positional inaccuracy set to 1 (equal to the padding option used in designing the barcodes). Benchmarking is performed in an 8-core machine with 24 Gb memory.