Transcriptome analyses of AR stimulated by 0.1 nM or 10 nM DHT in LNCaP stable cell line overexpressing AR [RNA-Seq]

The molecular drivers for the AR signaling reprogramming in castration-resistant prostate cancer remain to be determined. In this study, we hypothesize that increased AR expression in conjunction with lower-level androgens, which is a typical condition in prostate cancer cells receiving androgen deprivation therapy, is a major driving force of the reprogramming. To test this, we used LNCaP model with inducible overexpression of AR to examine the acute effects of AR overexpression stimulated by DHT on AR transcriptome. RNA-seq analyses were performed to examine gene expressions stimulated by 0.1 nM or 10 nM DHT with or without overexpression of AR in LNCaP stable cell line. The stable cells were generated by stably infecting LNCaP cells with tetracycline inducible overexpression of wild-type full length AR virus. These stable cells were grown in the medium containing 10% tetracycline-free FBS. The experiments were done in biological triplicates.