Maintenance of human hematopoietic stem cells in vitro using chemical cocktails

Single cell RNA-seq is a powerful tool for studying complex biological systems such as HSPCs cultured in vitro. We detected 11, 3, 4, 8 and 8 subpopulations in uncultured CD34+ cells,control cells, CD34+ cells with the treatment of CFO, cytokines and CFO + cytokines using t-Distributed Stochastic Neighbor Embedding (t-SNE) analysis. Overall design: We applied our homemade microwell single cell RNA-seq platform to perform analysis with uncultured human CD34+ cells, control cells, CFO cultured CD34+ cells, cytokine cultured CD34+ cells as well as CFO + cytokines cultured CD34+ cells.