Simulation results when releasing Wolbachia-infected mosquitoes in multiple batches.

Comparison of the number of days required to achieve 90% infection when releasing Wolbachia-infected mosquitoes at the threshold level (1,072,640 females and males) all at once or distributed in 2–5 weekly batches over an area with a mosquito dispersal rate of day. All five scenarios were evaluated with and without pre-release mitigation (Thermal Fogging 35%). The table shows the time to reach the threshold infection level for single-release and multi-batch mosquito releases. Pre-release mitigation significantly reduces the time to reach 90% infection, particularly for the single-release strategy, where thermal fogging reduces the days to 185 compared to 5 years without mitigation. Multi-batch releases (2–5 batches) generally show a quicker progression towards reaching 90% of infection, with the shortest times observed in the 5-batch release scenario, especially when combined with pre-release mitigation.