Analysis of cabazitaxel-resistant mechanism in human castration-resistant prostate cancer

To investigate cabazitaxel (CBZ) resistance in prostate cancer, we examined the changes in global gene expression before and after development of acquired CBZ resistance. Functional annotation clustering (FAC) analysis of DAVID showed cell division (GO: 0051301) and mitotic nuclear division (GO: 0007067) were the most enhanced clusters in PC3CR compared with PC3. PC3 and PC3CR cells were used for microarrays. PC3CR cells were newly established in our laboratory, which were grown and passaged upon reaching confluence in medium containing CBZ over a 18-month period.