Additional file 3 of Contrasting genetic variation and positive selection followed the divergence of NBS-encoding genes in Asian and European pears

Additional file 3. NBS-encoding genes in pear disease resistance QTL regions. A total of 18 QTL were detected from previous study [13, 16, 17, 22, 33–35]. The primers of SSR markers in QTL were used to detect the marker location on the reference genome [1, 32]. Markers upstream and downstream 500 kbp were selected to identify the presence of NBS genes related to these QTL.

附加文件3。梨抗病数量性状位点（Quantitative Trait Locus, QTL）区域内的NBS（核苷酸结合位点，Nucleotide-binding site）编码基因。既往研究[13, 16, 17, 22, 33–35]共检测到18个数量性状位点。本研究利用上述数量性状位点区域内的简单序列重复（Simple Sequence Repeat, SSR）标记引物，在参考基因组[1, 32]中定位该类标记的物理位置；随后选取各标记上下游各500千碱基对（kbp）的区域，以筛选与上述数量性状位点相关的NBS编码基因。