MOESM1 of Functional analysis of the role of CcpA in Lactobacillus plantarum grown on fructooligosaccharides or glucose: a transcriptomic perspective

Additional file 1: Figure S1. The sequencing saturation analysis of transcriptome data in the four conditions. Two replicate fermentations were carried out for each treatment and a total of 8 saturation curves were generated. Figure S2. Gene coverage distribution of transcriptome data in the four conditions. The percentage means the ratio of numbers of genes in different coverage intervals to the total number of genes. Figure S3. Representative volcano plots of six pair-wise comparisons. Table S1. Overview of whole transcriptome data in this study as determined by the RNA-seq analysis. Table S2. Summary of transcriptome data for wild-type and ccpA mutant strain grown on different carbon sources. Table S3. List of genes with putative cre sites that were significantly affected in the transcriptome analysis. Table S4. Local regulators subject to the regulation by CcpA. Table S5. Validation of transcriptome data by RT-qPCR on 18 selected genes. Table S6. Gene expression profiles of the key pathways in four pair-wise comparisons. Table S7. The gene expression of sacPTS1 and sacPTS26 gene clusters in four pair-wise comparisons. Table S8. The primers for RT-qPCR analysis with target gene information.

附加文件1：图S1。四种培养条件下转录组数据的测序饱和度分析。每组处理设置两次生物学重复发酵，共生成8条饱和度曲线。图S2。四种培养条件下转录组数据的基因覆盖度分布。此处百分比指不同覆盖度区间内的基因数占基因总数量的比例。图S3。六组两两比对的代表性火山图。表S1。本研究通过RNA测序（RNA-seq）分析得到的全转录组数据概况。表S2。不同碳源培养下野生型与ccpA突变菌株的转录组数据汇总。表S3。转录组分析中显著差异表达且推测带有cre位点的基因列表。表S4。受CcpA调控的局部调控因子一览表。表S5。通过实时定量聚合酶链式反应（RT-qPCR）对18个筛选基因进行转录组数据验证。表S6。四组两两比对中关键通路的基因表达谱。表S7。四组两两比对中sacPTS1与sacPTS26基因簇的基因表达情况。表S8。附带靶基因信息的实时定量PCR引物列表。