Interaction profiles of ACP mutants.

The two-hybrid interaction profiles have been obtained by assaying the interaction between the T18-ACP mutants and the indicated T25- constructions (first 4 columns), and also by assaying the T25-ACP mutants against T18-SpoT (middle column; Figure 4B). The co-purification results correspond to the experiment presented in figure 4A. The results obtained for the ACP/SpoT interaction in the three different assays (2-hybrid against T18-ACP, 2–hybrid against T25-ACP, and co-purification of SpoT-SG with T18-ACP on Calmodulin beads) are indicated.