Changes in nitrogen and phosphorus availability driven by secondary succession in temperate forests shape soil fungal communities and function

Soil fungal community plays an important role in forest ecosystems, and forest secondary succession is a crucial driver of soil fungal community. However, the driving factors of fungal community and function during temperate forest succession and their potential impact on succession processes are poorly understood. In this study, we investigated the dynamics of the soil fungal community in three temperate forest secondary successional stages (shrublands, coniferous forests, and deciduous broadleaf forests) using high-throughput DNA sequencing coupled with functional prediction via the FUNGuild database. We found that fungal community richness, α-diversity, and evenness decreased significantly during the succession process. Soil available phosphorus and nitrate nitrogen decreased significantly after initial succession occurred, and redundancy analysis showed that both were significant predictors of soil fungal community structure. Among functional groups, fungal saprotrophs as well as pa..., The soil samples were collected on 28 April 2021. There were three monodominant forest stands per forest type, each with a size of 100 × 100 m. Five individuals with similar diameter at breast high were chosen from each plot. Three soil subsamples were collected at a depth of 0–10 cm after litter removal, observing 1 m distance from the central tree in three directions at 120° angles. All five individual subsamples were homogenized to a single sample (Qu et al., 2020). Care was taken during tree selection to maintain at least a 10 m distance from non-target tree species, and each targeted tree was located farther than 20 m from the forest edge. A distance of at least 10 m was maintained between sampled trees to ensure spatial independence.
All samples were put on ice and transported back to the laboratory. After removing impurities (stones, roots), each sample was divided into three subsamples. (1) One subsample was immediately air-dried for determining soil pH, TN, total carbon (TC), t...,

土壤真菌群落（soil fungal community）在森林生态系统中发挥着关键作用，而森林次生演替是驱动土壤真菌群落演变的核心因素。然而，当前对于温带森林演替过程中真菌群落及其功能的驱动因子，以及其对演替进程的潜在影响，仍缺乏深入认知。本研究以温带森林次生演替的三个阶段（灌丛、针叶林、落叶阔叶林）为研究对象，采用高通量DNA测序结合FUNGuild数据库进行功能预测的方法，解析了土壤真菌群落的动态变化特征。研究发现，在演替过程中，真菌群落的丰富度、α多样性以及均匀度均呈显著下降趋势。初始演替完成后，土壤有效磷与硝态氮含量显著降低；冗余分析（redundancy analysis）结果显示，二者均为调控土壤真菌群落结构的重要预测因子。在真菌功能类群中，腐生真菌（fungal saprotrophs）以及……
土壤样品采集于2021年4月28日。每种森林类型设置3个单优林分样地，每个样地面积为100×100米。从每个样地中选取5株胸径（diameter at breast high）相近的目标树木。以每株目标树木为中心，在120°夹角的三个方向上距离树干1米处，去除地表枯落物后采集0–10 cm深度的土壤亚样品；将单株树木对应的5份土壤亚样品混匀为1份复合样品（Qu等，2020）。树木筛选过程中需确保目标树木与非目标树木间距至少10米，且每株目标树木距离林缘超过20米；采样树木间的间距需至少保持10米，以保证空间独立性。
所有样品置于冰盒中运输至实验室。去除样品中的杂质（石块、根系）后，将每份样品分为3份亚样品：(1) 其中1份亚样品立即进行风干处理，用于测定土壤pH值、总氮（TN）、总碳（TC）以及……