Additional file 1 of Genome-wide identification and expression pattern analysis of R2R3-MYB transcription factor gene family involved in puerarin biosynthesis and response to hormone in Pueraria lobata var. thomsonii

Additional file 1: Table S1. MYB-binding domain of PtMYBs. Table S2. Details information of the identified PtR2R3-MYB genes. Table S3. The prediction of subcellular localization in PtR2R3-MYB proteins. Table S4. Duplication modes and Ka/Ks ratios of R2R3-MYB gene pairs in P. thomsonii. Table S5. Putative functions of the R2R3-MYB proteins in P. thomsonii and Arabidopsis. Table S6. Detailed information for the 10 motifs in the R2R3-MYB proteins of P. thomsonii. Table S7a. Correlation between puerarin contents, SGs, PtR2R3-MYBs by RNA-seq data in P. thomsonii. Table S7b. P values between puerarin contents, SGs, PtR2R3-MYBs by RNA-seq data in P. thomsonii. Table S8. Structural genes related to puerarin biosynthesis. Table S9a. Correlation between puerarin and 46 genes by qRT-PCR data in 9 samples. Table S9b. P values between puerarin and 46 genes by qRT-PCR data in 9 samples. Table S10a. Correlation between puerarin and 46 genes by qRT-PCR data in ZG11. Table S10b. P values between puerarin and 46 genes by qRT-PCR data in ZG11. Table S11a. Correlation between puerarin and 46 genes by qRT-PCR data in ZG39. Table S11b. P values between puerarin and 46 genes by qRT-PCR data in ZG39. Table S12a. Correlation between puerarin and 46 genes by qRT-PCR data in ZG19. Table S12b. P values between puerarin and 46 genes by qRT-PCR data in ZG19. Table S13. Prediction of cis-acting elements in the promoter regions of 44 genes. Table S14a. Correletion between 44 genes qRT-PCR data by GSH treatment in ZG11. Table S14b. P values between 44 genes qRT-PCR data by GSH treatment in ZG11. Table S15a. Correletion between 44 genes qRT-PCR data by MeJA treatment in ZG11. Table S15b. P values between 44 genes qRT-PCR data by MeJA treatment in ZG11. Table S16. Primer sequence for qRT-PCR. Table S17. Puerarin contents and gene expression level by qRT-PCR.

附加文件1：表S1。PtMYBs的MYB结合结构域。表S2。已鉴定的PtR2R3-MYB基因的详细信息。表S3。PtR2R3-MYB蛋白的亚细胞定位预测。表S4。P. thomsonii中R2R3-MYB基因对的复制模式及Ka/Ks比值。表S5。P. thomsonii与拟南芥（Arabidopsis）中R2R3-MYB蛋白的推定功能。表S6。P. thomsonii的R2R3-MYB蛋白中10个基序的详细信息。表S7a。基于P. thomsonii RNA测序数据的葛根素含量、SGs与PtR2R3-MYBs的相关性。表S7b。基于P. thomsonii RNA测序数据的葛根素含量、SGs与PtR2R3-MYBs相关性的P值。表S8。与葛根素生物合成相关的结构基因。表S9a。基于9个样本的实时定量逆转录聚合酶链反应（qRT-PCR）数据的葛根素与46个基因的相关性。表S9b。基于9个样本的qRT-PCR数据的葛根素与46个基因相关性的P值。表S10a。基于ZG11样本的qRT-PCR数据的葛根素与46个基因的相关性。表S10b。基于ZG11样本的qRT-PCR数据的葛根素与46个基因相关性的P值。表S11a。基于ZG39样本的qRT-PCR数据的葛根素与46个基因的相关性。表S11b。基于ZG39样本的qRT-PCR数据的葛根素与46个基因相关性的P值。表S12a。基于ZG19样本的qRT-PCR数据的葛根素与46个基因的相关性。表S12b。基于ZG19样本的qRT-PCR数据的葛根素与46个基因相关性的P值。表S13。44个基因启动子区域的顺式作用元件预测。表S14a。基于ZG11样本经谷胱甘肽（GSH）处理后的44个基因qRT-PCR数据的相关性。表S14b。基于ZG11样本经GSH处理后的44个基因qRT-PCR数据相关性的P值。表S15a。基于ZG11样本经茉莉酸甲酯（MeJA）处理后的44个基因qRT-PCR数据的相关性。表S15b。基于ZG11样本经MeJA处理后的44个基因qRT-PCR数据相关性的P值。表S16。qRT-PCR引物序列。表S17。基于qRT-PCR的葛根素含量与基因表达水平。