Table2_Integrated Transcriptomics and Metabolomics Analyses of Stress-Induced Murine Hair Follicle Growth Inhibition.xlsx

Psychological stress plays an important role in hair loss, but the underlying mechanisms are not well-understood, and the effective therapies available to regrow hair are rare. In this study, we established a chronic restraint stress (CRS)-induced hair growth inhibition mouse model and performed a comprehensive analysis of metabolomics and transcriptomics. Metabolomics data analysis showed that the primary and secondary metabolic pathways, such as carbohydrate metabolism, amino acid metabolism, and lipid metabolism were significantly altered in skin tissue of CRS group. Transcriptomics analysis also showed significant changes of genes expression profiles involved in regulation of metabolic processes including arachidonic acid metabolism, glutathione metabolism, glycolysis gluconeogenesis, nicotinate and nicotinamide metabolism, purine metabolism, retinol metabolism and cholesterol metabolism. Furthermore, RNA-Seq analyses also found that numerous genes associated with metabolism were significantly changed, such as Hk-1, in CRS-induced hair growth inhibition. Overall, our study supplied new insights into the hair growth inhibition induced by CRS from the perspective of integrated metabolomics and transcriptomics analyses.

心理压力在脱发中扮演着至关重要的角色，但其潜在的机制尚未得到充分理解，且能够有效促进毛发再生的疗法寥寥无几。在本研究中，我们构建了一种慢性束缚应激（CRS）诱导的毛发生长抑制小鼠模型，并对其代谢组学和转录组学进行了全面分析。代谢组学数据分析揭示了CRS组皮肤组织中碳水化合物代谢、氨基酸代谢和脂质代谢等主要和次级代谢途径发生了显著改变。转录组学分析亦显示，参与代谢过程调节的基因表达谱，包括花生四烯酸代谢、谷胱甘肽代谢、糖酵解与糖异生、烟酸和烟酰胺代谢、嘌呤代谢、视黄醇代谢及胆固醇代谢等，均发生了显著变化。此外，RNA-Seq分析还发现，与代谢相关的众多基因，如Hk-1，在CRS诱导的毛发生长抑制过程中发生了显著变化。总体而言，本研究从代谢组学和转录组学综合分析的角度，为慢性束缚应激引起的毛发生长抑制提供了新的见解。