Additional file 6 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution

Additional file 6: Table S5. Results of tick and tick cell line screening for ASFLI-elements by qPCR. DNA from single ticks and every tick cell line (n=1) was extracted and analysed by qPCR for six ASFV-like genes as described in the ‘Material and methods’ section. All samples were tested with a qPCR control targeting a tick housekeeping gene to demonstrate successful DNA extraction and presence of tick DNA. Absence of ASFV in samples producing false positive results was proven by an OIE-listed qPCR.

补充材料6：表S5。通过实时荧光定量PCR（quantitative real-time PCR，qPCR）筛选蜱及蜱细胞系中非洲猪瘟样因子（ASFLI-elements）的结果。本研究按照"材料与方法"部分所述方法，提取单只蜱虫及每株蜱细胞系（样本量n=1）的基因组DNA，通过qPCR对6个非洲猪瘟病毒样基因进行检测分析。所有样本均采用靶向蜱持家基因的qPCR内参进行检测，以验证DNA提取成功且样本中存在蜱源DNA。针对出现假阳性结果的样本，采用世界动物卫生组织（Office International des Epizooties，OIE）收录的qPCR方法验证，证实其中不含非洲猪瘟病毒。